6-Methylsulfinylhexyl Isothiocyanate Inhibits Cell Cycle Progression in Quiescent Jb6 Cells Stimulated with Epidermal Growth Factor
نویسندگان
چکیده
Objective: The effects of 6-MSITC on cell cycle progression were investigated in quiescent mouse epidermal JB6 cells.
 Background: 6-Methylsulfinylhexyl isothiocyanate (6-MSITC) derived from wasabi (Wasabia japonica) has been reported to prevent tumor development vivo.
 Material and methods: Treatment with growth factor (EGF) cells, which serum-starved for 36 h, promoted the G0/G1 phase S phase. Effects pretreatment estimated by flowcytometry real-time RT-PCR.
 Results: Pretreatment at 0.25-1.0 μg/ml prior stimulation EGF significantly inhibited progression. gene expression DNA synthesis-related proteins cyclin A2, dumbbell former 4, proliferating nuclear antigen.
 Conclusion: These results showed that inhibits accompanied inhibition synthesis proteins.
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ژورنال
عنوان ژورنال: Annual research & review in biology
سال: 2021
ISSN: ['2347-565X']
DOI: https://doi.org/10.9734/arrb/2021/v36i630386